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  • Featured Product
  • KD/KO Validated

HBS1L Monoclonal antibody

HBS1L Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A10C4

Cat no : 68146-1-Ig

Synonyms

DKFZp686L13262, EF 1a, ERFS, HBS1, HBS1 like (S. cerevisiae), HBS1 like protein, HBS1L, HSPC276, KIAA1038

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis of HEK-293 using 68146-1-Ig

    WB result of HBS1L antibody (68146-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HBS1L transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis using 68146-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of various lysates using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis using 68146-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using HBS1L Monoclonal antibody (68146-1-Ig)

    WB analysis of HepG2 using 68146-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68146-1-Ig (HBS1L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HBS1L Monoclonal antibody (68146-1-Ig)

    IF Staining of U2OS using 68146-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HBS1L Monoclonal antibody (68146-1-Ig)

    IF Staining of U2OS using 68146-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using HBS1L antibody (68146-1-Ig, Clone: 1A10C4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inNCI-H1299 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, LNCaP cells, HeLa cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68146-1-Ig targets HBS1L in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HBS1L fusion protein Ag0388
Full Name HBS1-like (S. cerevisiae)
Calculated Molecular Weight 75 kDa
Observed Molecular Weight75 kDa
GenBank Accession NumberBC001465
Gene Symbol HBS1L
Gene ID (NCBI) 10767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HBS1L (HBS1-like), also known as EF-1a or ERFS, is a 684 amino acid protein that belongs to the GTP-binding elongation factor family and exists as multiple alternatively spliced isoforms. Expressed in kidney, brain, heart, placenta, liver, muscle and pancreas, HSB1L is thought to play a role in controlling fetal hemoglobin levels, specifically influencing platelet, monocyte and erythrocyte hemoglobin content. The gene encoding HBS1L maps to a locus on human chromosome 6 that is associated with sickle cell anemia and beta-thalassemia, suggesting a role for HBS1L in the pathogenesis of blood disorders.This antibody specifically recognizes the 75kd human HBS1L protein.

Protocols

Product Specific Protocols
WB protocol for HBS1L antibody 68146-1-IgDownload protocol
IF protocol for HBS1L antibody 68146-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols