HBE1-Specific Monoclonal antibody, PBS Only

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  WB analysis of K-562 using 66151-1-Ig (same clone as 66151-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66151-1-Ig (HBE1-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66151-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 66151-1-Ig (same clone as 66151-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66151-1-Ig (HBE1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66151-1-PBS in a different storage buffer formulation.

HBE1-Specific Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66151-1-PBS

Clone No.2C11G6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Epsilon globin, HBE, HBE1, HBE1-Specific, Hemoglobin epsilon, Hemoglobin epsilon chain, Hemoglobin epsilon-Specific, Hemoglobin subunit epsilon, hemoglobin, epsilon 1

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Product Information

66151-1-PBS targets HBE1-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: hemoglobin, epsilon 1
• Observed Molecular Weight: 16 kDa
• GenBank Accession Number: NM_005330
• Gene Symbol: HBE1
• Gene ID (NCBI): 3046
• RRID: AB_2881547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P02100
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

The hemoglobin molecule is a tetramer consisting of two α-globin-like polypeptide chains and two β-globin-like chains. The human hemoglobin genes are expressed in a tightly developmentally controlled fashion.ε-globin (HBE1) is the predominantly expressed gene during the embryonic stage. The epsilon hemoglobin chain seems to be the best marker for fetal nucleated red blood cells (NRBCs). Anti-HBE1 may be used to label and isolate fetal cells from maternal blood and can be useful in prenatal diagnosis. This antibody specifically recognizes the HBE1 and doesn't cross-react with other globin chains.