Hemoglobin Alpha Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP00239-3

  Sandwich ELISA standard curve of MP00239-3, Human Hemoglobin alpha Recombinant Matched Antibody Pair - PBS only. 83185-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag6040.83185-3-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP00239-3

  The mean Hemoglobin alpha concentration was determined to be 40.7 μg/mL in Human CSF

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  Sample test of MP00239-3

  Serum of fifteen individual healthy human was measured. The Hemoglobin alpha concentration of detected samples was determined to be 41.2 pg/mL with a range of 14.8-96.6 ng/mL.

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  Cytometric bead array standard curve of MP00239-1

  Cytometric bead array standard curve of MP00239-1, Hemoglobin alpha Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83185-3-PBS. Detection antibody: 83185-4-PBS. Standard: Ag6040. Range: 3.125-100 ng/mL.

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  Affinity and Kinetic Characterization of 83185-4-PBS

  Biolayer interferometry (BLl) kinetic assay of 83185-4-PBS against Human Hemoglobin Alpha was performed. The affinity constant is 2.55 nM.

Hemoglobin Alpha Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83185-4-PBS

Clone No.240049H3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

HBA1, Hemoglobin a, HBA2, Alpha-globin, Alpha globin

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Product Information

83185-4-PBS targets Hemoglobin Alpha as part of a matched antibody pair:

MP00239-1: 83185-3-PBS capture and 83185-4-PBS detection (validated in Cytometric bead array)

MP00239-3: 83185-4-PBS capture and 83185-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Hemoglobin Alpha fusion protein Ag6040
• Full Name: hemoglobin, alpha 1
• Calculated Molecular Weight: 15 kDa
• GenBank Accession Number: BC050661
• Gene Symbol: HBA1
• Gene ID (NCBI): 3039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P69905
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.