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HAPLN1 Recombinant antibody, PBS Only

HAPLN1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241588E9

Cat no : 84374-4-PBS

Synonyms

Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAPLN1 Recombinant antibody (84374-4-RR)

    WB analysis using 84374-4-RR (same clone as 84374-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84374-4-RR (HAPLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84374-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84374-4-RR

    Affinity and Kinetic Characterization of 84374-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84374-4-RR against Human HAPLN1 were performed. The affinity constant is below 1 pM.

Product Information

The immunogen of 84374-4-PBS is HAPLN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HAPLN1 fusion protein Ag25221
Full Name hyaluronan and proteoglycan link protein 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC057808
Gene Symbol HAPLN1
Gene ID (NCBI) 1404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453). It is reported in the literature that it is a double band.(PMID: 26341258)