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HAPLN1 Recombinant antibody

HAPLN1 Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241588E9

Cat no : 84374-4-RR

Synonyms

Proteoglycan link protein, CRTL1, CRT1, Cartilage-linking protein 1, Cartilage-link protein

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HAPLN1 Recombinant antibody (84374-4-RR)

    WB analysis using 84374-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84374-4-RR (HAPLN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 84374-4-RR is HAPLN1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HAPLN1 fusion protein Ag25221
Full Name hyaluronan and proteoglycan link protein 1
Calculated Molecular Weight 40 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberBC057808
Gene Symbol HAPLN1
Gene ID (NCBI) 1404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HAPLN1 (hyaluronan and proteoglycan link protein 1), also known as CRTL1. It is expected to be located in the cytoplasm and extracellular, and the protein is mainly expressed in placenta and small intestine. The calculated molecular weight of HAPLN1 is 40 kDa. HAPLN1 binds to aggrecan on the hyaluronic chain, stabilizing the aggregates and thus increasing compression resistance and shock absorption. HAPLN1 also functions as a growth factor, up regulating aggrecan and type II collagen synthesis in cartilage. It seems to be an essential part of cartilage homeostasis and may also contribute to homeostasis in the disc tissue (PMID: 23537453). It is reported in the literature that it is a double band.(PMID: 26341258)

Protocols

Product Specific Protocols
WB protocol for HAPLN1 antibody 84374-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols