ChromoTek HA-Trap Magnetic Agarose

The ChromoTek HA-Trap Magnetic Agarose consists of an anti-HA-tag Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of HA-fusion proteins from cell extracts of various organisms such as humans, mice, dogs, plants, and yeast.

Specificity

HA-Tag (sequence YPYDVPDYA)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Cat no : atma

Synonyms

Hemagglutinin tag, HA, HA-tag, HA-tag Trap Magnetic Agarose



Product Information

The ChromoTek HA-Trap Magnetic Agarose consists of an anti-HA-tag Nanobody/VHH, which is coupled to magnetic agarose beads. It can be used for the immunoprecipitation of HA-fusion proteins from cell extracts of various organisms such as humans, mice, dogs, plants, and yeast.

DescriptionImmunoprecipitation of HA-fusion proteins with anti-HA Nanobody conjugated to magnetic agarose beads.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

• Works in samples from: mammals, plants, yeast, etc.

ApplicationsIP, Co-IP
Specificity/TargetBinds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
Binding Capacity20 ug recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry
ConjugateMagnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
Elution buffer2x SDS-sample buffer (Lammli)
Wash Buffer Compatibility2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
TypeNanobody
ClassRecombinant
HostAlpaca
Affinity (KD) 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions.
Compatibility with mass spectrometryThe HA-Trap is optimized for on-bead digestion. For the application note, please click here:
On-bead digest protocol for mass spectrometry
RRIDAB_3094561
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Publications

ApplicationTitle
IP

Plant Physiol

ABSCISIC ACID-INSENSITIVE 5-KIP-RELATED PROTEIN 1-SHOOT MERISTEMLESS modulates reproductive development of Arabidopsis

Authors - Wan-Ni Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sandra (Verified Customer) (03-28-2024)

The HA-magnetic beads enriched my low abundant bait protein in a higher yield compared to other HA-magnetic beads from another company and in addition with lower background impurities. Elution was performed with 3xHA-peptide and Laemmli buffer.

  • Applications: Immunoprecipitation
  • Cell Tissue Type: Saccharomyces cerevisiae