ChromoTek HA-Trap Magnetic Agarose Kit

The HA-Trap Magnetic Agarose Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to magnetic agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

Specificity

HA-Tag (sequence YPYDVPDYA)

Applications

IP, Co-IP

Type

Nanobody

Conjugate

Magnetic Agarose

Cat no : atmak

Synonyms

Hemagglutinin tag, HA, HA-tag, HA-tag Trap Magnetic Agarose

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Validation Data Gallery

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  The HA-Trap Magnetic Agarose Kit was used to immunoprecipitate TOM70-HA fusion protein from either untransfected (mock) HEK293T cells or HEK293T cell transfected with full-length TOM70-HA construct. SDS-PAGE analysis was done on samples from the Input (I), Flow-through (F), Bound (B) fractions.

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  Co-IP using HA-Trap Magnetic Agarose Kit followed by multiplexed WB of TOM70-HA and HSP90 proteins from untransfected (mock) HEK293T cells and HEK293T cells transfected with full-length TOM70-HA construct. WB analysis was done on samples from the Input (I), Flow-through (F) and Bound (B) fractions of the IP. TOM70 Monoclonal Antibody (66593-1-Ig), Multi-rAB CoraLite Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM002), HSP90 Polyclonal Antibody (13171-1-AP), and Multi-rAb CoraLite Plus 750-Goat Anti Rabbit Recombinant Secondary Antibody (RGAR006) were used in the WB analysis.

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Product Information

The HA-Trap Magnetic Agarose Kit is a ready-to-use reagent for the IP of HA-tagged proteins. It consists of an anti-HA-tag Nanobody/VHH coupled to magnetic agarose beads, along with lysis, wash, and elution buffers to use in the IP process.

• Description: The HA-Trap Magnetic Agarose Kit contains HA-Trap magnetic agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of HA-tagged proteins.;

  • Fast, reliable & efficient one-step immunoprecipitation;

  • Ready-to-use;

  • No heavy & light antibody chains;

  • Stable under harsh washing conditions;

  • Suitable for downstream mass spec analysis;

  • Works in samples from: mammals, plants, yeast, etc.

• Applications: IP, Co-IP
• Specificity/Target: Binds specifically to the HA-tag (sequence YPYDVPDYA) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion. There is no cross-reactivity to other common peptide tags such as the His6-tag, FLAG-tag, Spot-Tag, V5-tag, Strep-tag, or C-tag (other tags not tested). Background binding to host cell proteins from a range of organisms such as human, mouse and dog cell lines or yeast and plants is low.
• Binding Capacity: 20 ug of recombinant HA-tagged protein (~30 kDa) per 25 uL bead slurry
• Conjugate: Magnetic agarose beads; ~40 um (cross-linked 6% magnetic agarose beads)
• Elution buffer: 2x SDS-sample buffer (Lammli)
• Wash Buffer Compatibility: 2M NaCl, 5 mM DTT, 0 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, 0% SDS, 2-3 M Urea
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): 6 nM for C-terminal HA-tags and ca. 180 nM for N-terminal fusions.
• Compatibility with mass spectrometry: The HA-Trap is optimized for on-bead digestion. For the application note, please click here:; On-bead digest protocol for mass spectrometry
• RRID: AB_3094561
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. DO not freeze!

Kit components

Component	Description
HA-Trap Magnetic Agarose	20 reactions (500 µl)
Lysis buffer	Optimized for cytoplasmic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution