HRP-conjugated HA Tag Recombinant antibody

HA Tag Uni-rAb^TM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (1)

Applications

WB, IP, CoIP

Conjugate

HRP

Publications

5

CloneNo.

6I21

Cat no : HRP-81290

Synonyms

HA

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Validation Data Gallery

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  WB analysis of Transfected HEK-293T using HRP-81290

  Non-transfected and transfected HEK-293T lysates were subjected to SDS PAGE followed by western blot with HRP-81290 (HA tagged antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Transfected HEK-293T cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

HRP-81290 targets HA Tag in WB, IP, CoIP applications and shows reactivity with recombinant protein samples.

• Tested Reactivity: recombinant protein
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: HA Tag
• Conjugate: HRP
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.

Publications

Species	Application	Title
	WB	Int J Mol Sci; CircMTA2 Drives Gastric Cancer Progression through Suppressing MTA2 Degradation via Interacting with UCHL3; Authors - Gengchen Xie
human	WB,CoIP	Cell Rep; A pro-metastatic tRNA fragment drives aldolase A oligomerization to enhance aerobic glycolysis in lung adenocarcinoma; Authors - Qinglin Wang
human	WB	PLoS One; USP26 suppresses type I interferon signaling by targeting TRAF3 for deubiquitination; Authors - Cheng-Lan Sheng
	WB,IP	Heliyon; Nuclear translocation of nucleotide enzyme Phosphoglucomutase 2 governs DNA damage response and anti-tumor immunity; Authors - Yingying Lyu
	WB	Int J Mol Sci; A Combined Transcriptomic and Proteomic Analysis of Monkeypox Virus A23 Protein on HEK293T Cells; Authors - Yihao Wang