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HA Tag Recombinant antibody, PBS Only

HA Tag Recombinant Antibody for WB, IP, IF, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein

Applications

WB, IP, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6I21

Cat no : 81290-1-PBS

Synonyms

HA, HA Tag

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HA Tag Recombinant antibody (81290-1-RR)

    WB analysis using 81290-1-RR (same clone as 81290-1-PBS)

    HA-tag recombinant protein, non-transfected HEK-293 cells, transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 81290-1-RR (HA Tag antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Transfected HEK-293T cells using HA Tag Recombinant antibody (81290-1-RR)

    IP experiment of Transfected HEK-293T using 81290-1-RR (same clone as 81290-1-PBS)

    IP result of anti-HA Tag (IP:81290-1-RR, 4ug; Detection:81290-1-RR 1:20000) with Transfected HEK-293T cells lysate 795 ug. This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using HA Tag Recombinant antibody (81290-1-RR)

    IF Staining of Transfected HEK-293 using 81290-1-RR (same clone as 81290-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using HA Tag antibody (81290-1-RR, Clone: 6I21 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81290-1-PBS in a different storage buffer formulation.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81290-1-PBS targets HA Tag in WB, IP, IF, Indirect ELISA applications and shows reactivity with recombinant protein samples.

Tested Reactivity recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name HA Tag
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Protein tags are a protein or peptide sequences located either on the C- or N- terminal of the target protein, which can facilitate solubility, detection, purification, localization, and expression of the target protein. The HA tag corresponds to amino acid residues YPYDVPDYA of a surface glycoprotein -human influenza virus hemagglutinin (HA). The HA tag is commonly used for a variety of research applications including chromatin immunoprecipitation, ELISA, flow cytometry, western blotting, immunocytochemistry/immunofluorescence among of others.