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Granzyme A Recombinant antibody, PBS Only

Granzyme A Uni-rAbTM Recombinant Antibody for IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241979B8

Cat no : 84583-3-PBS

Synonyms

GrA, GZMA, Fragmentin 1, EC:3.4.21.78, Cytotoxic T-lymphocyte proteinase 1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using Granzyme A Recombinant antibody (84583-3-RR)

    IF Staining of Jurkat using 84583-3-RR (same clone as 84583-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using GZMA antibody (84583-3-RR, Clone: 241979B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84583-3-PBS in a different storage buffer formulation.

Product Information

The immunogen of 84583-3-PBS is Granzyme A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Granzyme A fusion protein Ag1818
Full Name granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3)
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC015739
Gene Symbol Granzyme A
Gene ID (NCBI) 3001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Granzyme A(GrA, a tryptase) is necessary for target cell lysis in cell-mediated immune responses. It activates mitochondrial outer membrane permeabilization- and caspase-independent cell death pathways by cleaving the mitochondrial complex I component NDUFS3after lys56. Among serine proteases GrA has an unique quaternary structure consisting of a disulphide-linked homodimer of 60kDa linked via Cys93.(PMID: 12819769) This protein has 2 isoforms produced by alternative promoter usage with the MW of 29 and 27 kDa.