Biotin-conjugated Progranulin/PGRN Polyclonal antibody

• Featured Product
• KD/KO Validated

Progranulin/PGRN Polyclonal Antibody for IHC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Conjugate

Biotin

Cat no : Biotin-18410

Synonyms

GEP, GP88, Granulin, GRN, Progranulin

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Validation Data Gallery

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  IHC staining of human kidney using Biotin-18410

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-18410(Granulin antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using Biotin-18410

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using Biotin-18410(Granulin antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-18410 targets Progranulin/PGRN in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Progranulin/PGRN fusion protein Ag13178
• Full Name: granulin
• Calculated Molecular Weight: 64 kDa
• GenBank Accession Number: BC000324
• Gene Symbol: Granulin
• Gene ID (NCBI): 2896
• RRID: AB_2883060
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.03% Proclin300, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. This antibody is conjugated with Biotin.