Glypican 2 Recombinant antibody

Glypican 2 Uni-rAbTM Recombinant Antibody for ELISA
Cat No. 83241-1-RR
Clone No.230518A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA

GPC2, Secreted glypican-2, FLJ38962, 230518A4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive FC (Intra) detected inSH-SY5Y cells, HeLa cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83241-1-RR targets Glypican 2 in ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD<1.00 x 10-12M
KOff<1.00 x 10-6M
KOn=1.07 x 105M
Immunogen Glypican 2 fusion protein Ag4041 Predict reactive species
Full Name glypican 2
Calculated Molecular Weight 579 aa, 63 kDa
Observed Molecular Weight62,70-100 kDa
GenBank Accession NumberBC027972
Gene Symbol GPC2
Gene ID (NCBI) 221914
RRIDAB_3670917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8N158
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).

Protocols

Product Specific Protocols
FC protocol for Glypican 2 antibody 83241-1-RRDownload protocol
Standard Protocols
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