Glypican 2 Recombinant antibody

Glypican 2 Uni-rAb^TM Recombinant Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

ELISA

Conjugate

Unconjugated

CloneNo.

230518A4

Cat no : 83241-1-RR

Synonyms

GPC2, Secreted glypican-2, FLJ38962, 230518A4

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Validation Data Gallery

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  FC experiment of HeLa using 83241-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of SH-SY5Y using 83241-1-RR

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83241-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive FC (Intra) detected in: SH-SY5Y cells, HeLa cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83241-1-RR targets Glypican 2 in ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D<1.00 x 10^-12M
  K_Off<1.00 x 10^-6M
  K_On=1.07 x 10^5M
• Immunogen: Glypican 2 fusion protein Ag4041
• Full Name: glypican 2
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 62，70-100 kDa
• GenBank Accession Number: BC027972
• Gene Symbol: GPC2
• Gene ID (NCBI): 221914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).