Glypican 2 Recombinant antibody, PBS Only (Capture)

Glypican 2 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230518A4

Cat no : 83241-1-PBS

Synonyms

GPC2, Secreted glypican-2, FLJ38962, 230518A4

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Validation Data Gallery

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  FC experiment of HeLa using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 83241-1-RR (same clone as 83241-1-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Gpc2 Recombinant Antibody (83241-1-RR, Clone:230518A4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83241-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP00156-2

  Cytometric bead array standard curve of MP00156-2, GPC2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83241-1-PBS. Detection antibody: 83241-2-PBS. Standard: Ag4041. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 83241-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83241-1-RR against Human Glypican 2 were performed. The affinity constant is below 1 pM.

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Product Information

83241-1-PBS targets Glypican 2 as part of a matched antibody pair:

MP00156-2: 83241-1-PBS capture and 83241-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Affinity:
  K_D<1.00 x 10^-12M
  K_Off<1.00 x 10^-6M
  K_On=1.07 x 10^5M
• Immunogen: Glypican 2 fusion protein Ag4041
• Full Name: glypican 2
• Calculated Molecular Weight: 579 aa, 63 kDa
• Observed Molecular Weight: 62，70-100 kDa
• GenBank Accession Number: BC027972
• Gene Symbol: GPC2
• Gene ID (NCBI): 221914
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPC2 (glypican 2), which is expected to be located in cell membrane and extracellular space. It is expressed in lymphoid tissue, skin and testis. The calculated molecular weight of the protein is 62 kDa. GPC2 is mainly active in growing nervous tissues and thyroid cancer tissues (PMID: 28616017). It participates in the growth and differentiation of neuronal axons. Increasing evidence has demonstrated the overexpression of GPC2 in neuroblastoma, a kind of childhood cancer. There is a discovery that GPC2 can be employed as a diagnostic, prognostic, and immunological predictor of generalized cancers. The study may broaden the train of thought toward application of GPC2 in immunotherapy (PMID: 35345673).