Anti-Human Glycophorin A/CD235a Rabbit Recombinant Antibody

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  FC experiment of Human peripheral blood erythrocytes using 98370-2-RR

  1x10^6 Human peripheral blood erythrocytes were surface stained with 0.25 ug Anti-Human Glycophorin A/CD235a Rabbit RecAb (98370-2-RR, Clone:242822E1) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

Glycophorin A/CD235a Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98370-2-RR

Clone No.242822E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik

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Tested Applications

• Positive FC detected in: Human peripheral blood erythrocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98370-2-RR targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: glycophorin A (MNS blood group)
• Calculated Molecular Weight: 150 aa, 16 kDa
• GenBank Accession Number: BC005319
• Gene Symbol: Glycophorin A
• Gene ID (NCBI): 2993
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02724
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)