CoraLite®594-conjugated Glutamine Synthetase Monoclonal antibody

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• KD/KO Validated

Glutamine Synthetase Monoclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6G4

Cat no : CL594-66323

Synonyms

GLUL, Glutamate--ammonia ligase, GLNS, EC:6.3.1.2, EC:2.3.1.225

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323, Clone: 1B6G4 ) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-66323

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 Glutamine Synthetase antibody (CL594-66323-2, Clone: 1B6G4 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-66323

  1X10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human Glutamine Synthetase (CL594-66323, Clone:1B6G4) (red), or 0.8 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse brain tissue
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66323 targets Glutamine Synthetase in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Full Name: glutamate-ammonia ligase (glutamine synthetase)
• Calculated Molecular Weight: 374 aa, 42 kDa
• GenBank Accession Number: BC011700
• Gene Symbol: Glutamine Synthetase
• Gene ID (NCBI): 2752
• RRID: AB_2883558
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.