Glutamine Synthetase Monoclonal antibody, PBS Only

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• KD/KO Validated

Glutamine Synthetase Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1D10G8

Cat no : 66323-1-PBS

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

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Validation Data Gallery

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  WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

  WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 66323-1-Ig (same clone as 66323-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  WB analysis of pig liver using 66323-1-Ig (same clone as 66323-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66323-1-Ig (same clone as 66323-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  IF Staining of zebrafish retina using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK. This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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  IF Staining of human brain using 66323-1-Ig (same clone as 66323-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66323-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66323-1-PBS targets Glutamine Synthetase in WB, IF, IHC, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Glutamine Synthetase fusion protein Ag6309
• Full Name: glutamate-ammonia ligase (glutamine synthetase)
• Calculated Molecular Weight: 374 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC011700
• Gene Symbol: GLUL
• Gene ID (NCBI): 2752
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).