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  • Featured Product
  • KD/KO Validated

Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for WB, IHC, IF, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, zebrafish and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1D10G8

Cat no : 66323-1-Ig

Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of Jurkat using 66323-1-Ig

    WB result of Glutamine synthetase antibody (66323-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Glutamine synthetase transfected Jurkat cells.

  • Western Blot (WB) analysis of Jurkat cells using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of Jurkat using 66323-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of pig liver using 66323-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    WB analysis of pig brain using 66323-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human liver cancer using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human brain using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IHC staining of human brain using 66323-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human brain tissue using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IF Staining of human brain using 66323-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human brain tissue using 66323-1-Ig (Glutamine synthetase antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of zebrafish retina using Glutamine Synthetase Monoclonal antibody (66323-1-Ig)

    IF Staining of zebrafish retina using 66323-1-Ig

    Immunofluorescence analysis of the zebrafish retina at 5 days post fertilisation. Collagen18a1 (aka Endostatin) polyclonal antibody (18301-1-AP, magenta). Dilution 1:200. Glutamine synthetase (66323-1-Ig, green) dilution 1:500. Tissue fixed overnight in 4% PFA; DAPI, blue. Data generated by Natalia Jaroszynska in Professor Ryan MacDonald's lab, University College London, UK.

Tested Applications

Positive WB detected inJurkat cells, pig liver tissue, pig brain tissue
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inzebrafish retina, human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66323-1-Ig targets Glutamine Synthetase in WB, IHC, IF, ELISA applications and shows reactivity with human, pig, zebrafish samples.

Tested Reactivity human, pig, zebrafish
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Glutamine Synthetase fusion protein Ag6309
Full Name glutamate-ammonia ligase (glutamine synthetase)
Calculated Molecular Weight 374 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC011700
Gene Symbol Glutamine Synthetase
Gene ID (NCBI) 2752
RRIDAB_2881703
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts (PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). There are other bands with higher (66 kDa, 97 kDa) and lower (30 kDa)molecular weights also detected besides the 42 kDa band indicating the proteolysis of GLUL protein by the ubiquitin system(PMID:10091759).

Protocols

Product Specific Protocols
WB protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IHC protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
IF protocol for Glutamine Synthetase antibody 66323-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai
View Article
mouseIF

Acta Neuropathol

Selective vulnerability of tripartite synapses in amyotrophic lateral sclerosis.

Authors - Matthew J Broadhead
View Article

J Neurosci

C9ORF72 deficiency results in neurodegeneration in the zebrafish retina

Authors - Natalia Jaroszynska
View Article
zebrafishIF

Life Sci Alliance

Unique activities of two overlapping PAX6 retinal enhancers

Authors - Kirsty Uttley
View Article
ratIF

In Vitro Cell Dev Biol Anim

TRIM9 promotes Müller cell-derived retinal stem cells to differentiate into retinal ganglion cells by regulating Atoh7

Authors - Qi Zeng
View Article
mouseIF

PLoS One

Excitatory to inhibitory synaptic ratios are unchanged at presymptomatic stages in multiple models of ALS

Authors - Calum Bonthron
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Calum (Verified Customer) (11-02-2021)

Glutamine synthetase (magenta) alongside alternative astrocyte marker GFAP (green) and DAPI (blue).

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Primary Mouse Astrocytes
Glutamine Synthetase Antibody Immunofluorescence,Cell culture validation (1:500 dilution) in Primary Mouse Astrocytes (Cat no:66323-1-Ig)
FH

Ryan (Verified Customer) (02-27-2019)

Tris-HCL antigen retrieval ph=9.

  • Applications: Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish retina (Muller glia) at 3dpf
Glutamine Synthetase Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Muller glia) at 3dpf (Cat no:66323-1-Ig)