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CoraLite® Plus 488-conjugated Glucocorticoid receptor Recombinant antibody

Glucocorticoid receptor Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2F22

Cat no : CL488-82619-4

Synonyms

GCCR, GCR, Glucocorticoid receptor, GR, GRL, NR3C1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Glucocorticoid recep (CL488-82619-4)

    IF Staining of HepG2 using CL488-82619-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-82619-4, Clone: 2F22 ) at dilution of 1:200.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated Glucocorticoid recep (CL488-82619-4)

    IF Staining of HepG2 using CL488-82619-4

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 Glucocorticoid receptor antibody (CL488-82619-4, Clone: 2F22 ) at dilution of 1:200, CL594-phalloidin (red).

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82619-4 targets Glucocorticoid receptor in IF/ICC applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Glucocorticoid receptor fusion protein Ag21146
Full Name nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Calculated Molecular Weight 86 kDa
Observed Molecular Weight 94-97 kDa
GenBank Accession NumberBC015610
Gene Symbol Glucocorticoid receptor
Gene ID (NCBI) 2908
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucocorticoid receptor (GR, or GCR) also known as NR3C1 (nuclear receptor subfamily 3, group C, member 1) is a receptor for glucocorticoids, which owns a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. It is involved in cell proliferation and differentiation and specifically implicated in newborn birth weight, thus providing a biological mechanism by which NR3C1 expression may influence birth weight (PMID:22810058).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Glucocorticoid receptor antibody CL488-82619-4Download protocol
Standard Protocols
Click here to view our Standard Protocols