Tested Applications
Positive WB detected in | HT-29 cells, T-47D cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66445 targets Gamma Catenin in WB applications and shows reactivity with human, rat, pig, Canine samples.
Tested Reactivity | human, rat, pig, Canine |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Gamma Catenin fusion protein Ag1627 Predict reactive species |
Full Name | junction plakoglobin |
Calculated Molecular Weight | 82 kDa |
Observed Molecular Weight | 82 kDa |
GenBank Accession Number | BC011865 |
Gene Symbol | Gamma Catenin |
Gene ID (NCBI) | 3728 |
RRID | AB_3084247 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P14923 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.
Protocols
Product Specific Protocols | |
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WB protocol for CL Plus 488 Gamma Catenin antibody CL488-66445 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |