Gamma Catenin Monoclonal antibody, PBS Only

Gamma Catenin Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, rat, pig, Canine, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A5D10

Cat no : 66445-1-PBS

Synonyms

ARVD12, Catenin gamma, CTNNG, Desmoplakin 3, Desmoplakin III, DP3, DPIII, junction plakoglobin, JUP, PDGB, PKGB, γ-Catenin

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Validation Data Gallery

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  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HT-29 using 66445-1-Ig (same clone as 66445-1-PBS)

  HT-29 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  WB analysis of T-47D using 66445-1-Ig (same clone as 66445-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human heart using 66445-1-Ig (same clone as 66445-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 66445-1-Ig (same clone as 66445-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MDCK using 66445-1-Ig (same clone as 66445-1-PBS)

  MDCK cells were subjected to SDS PAGE followed by western blot with 66445-1-Ig (gamma catenin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66445-1-Ig (gamma catenin antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse stomach using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 66445-1-Ig (Gamma Catenin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 66445-1-Ig (Plakoglobin/gamma catenin antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 66445-1-Ig (same clone as 66445-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Gamma Catenin antibody (66445-1-Ig, Clone: 1A5D10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66445-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66445-1-PBS targets Gamma Catenin in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, rat, pig, Canine, mouse samples.

• Tested Reactivity: human, rat, pig, Canine, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Catenin fusion protein Ag1627
• Full Name: junction plakoglobin
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC011865
• Gene Symbol: JUP
• Gene ID (NCBI): 3728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Plakoglobin (γ-catenin), a member of the Armadillo proteins, plays important roles in cell adhesion with α-catenin and β-catenin, is involved in Wnt signaling, and is translocated into the nucleus and binds LEF1. Plakoglobin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. Plakoglobin links cadherins to the actin cytoskeleton. Mutations in plakoglobin are associated with arrhythmogenic right ventricular dysplasia and Pemphigus vulgaris.