Galectin-3 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Galectin-3 Monoclonal Antibody for WB, IF, FC, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 60207-1-PBS

Synonyms

35 kDa lectin, CBP 35, CBP35, Gal 3, GAL3, Galactose specific lectin 3, Galactoside binding protein, GALBP, Galectin 3, Galectin-3, GALIG, IgE binding protein, L 31, Laminin binding protein, Lectin L 29, LGALS2, LGALS3, Mac 2 antigen, MAC2

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Validation Data Gallery

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  WB analysis using 60207-1-Ig (same clone as 60207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa cells using 60207-1-Ig (same clone as 60207-1-PBS)

  WB result of Galectin-3 specific antibody (60207-1-Ig, 1:1000) with si-control and si-Galectin-3 transfected HeLa cells. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 60207-1-Ig (same clone as 60207-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 60207-1-Ig (Galectin-3 antibody at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IF Staining of human thyroid cancer using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human thyroid cancer tissue using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse kidney using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using Galectin-3 antibody (60207-1-Ig, Clone: 1C1B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 60207-1-Ig (same clone as 60207-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 60207-1-Ig (Galectin-3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 60207-1-Ig (same clone as 60207-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human Galectin-3 (60207-1-Ig, Clone:1C1B2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60207-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60207-1-PBS targets Galectin-3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Galectin-3 fusion protein Ag6753
• Full Name: lectin, galactoside-binding, soluble, 3
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC001120
• Gene Symbol: LGALS3
• Gene ID (NCBI): 3958
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-3, a member of the β-galactoside-binding proteins, contains one carbohydrate recognition domain (CRD) and a proline- and glycine-rich N-terminal domain through which is able to form oligomers (PMID: 14758078). Galectin-3 is widely expressed in many normal tissues and a variety of tumors. It is found intracellularly in nucleus and cytoplasm or secreted outside of cell, being present on the cell surface or in the extracellular space (PMID: 16478649). Galectin-3 is involved in various biological processes including cell growth, adhesion, differentiation, apoptosis, angiogenesis, immune response, neoplastic transformation and metastasis (PMID: 16478649; 14758078).