Mouse Galc Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Galc Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11991-1-PBS

Synonyms

---

Validation Data Gallery

• 

  WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 11991-1-AP (same clone as 11991-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11991-1-AP (Galc antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

• 

  IP experiment of NIH/3T3 using 11991-1-AP (same clone as 11991-1-PBS)

  IP result of anti-Galc (IP:11991-1-AP, 4ug; Detection:11991-1-AP 1:300) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 11991-1-AP (same clone as 11991-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11991-1-AP (Galc antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11991-1-PBS in a different storage buffer formulation.

---

Product Information

11991-1-PBS targets Galc in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galc fusion protein Ag3914
• Full Name: galactosylceramidase
• Calculated Molecular Weight: 77 kDa
• Observed Molecular Weight: 80 kDa, 30 kDa, 50 kDa
• GenBank Accession Number: BC086671
• Gene Symbol: Galc
• Gene ID (NCBI): 14420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

The GALC antibody targets the liposomal enzyme Galactosylceramidase (GALC), which belongs to the glycosyl hydrolase 59 family. It hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. It is primarily found in the brain and kidneys where galactolipids are hydrolyzed (PMID:8634707). Deficiencies of GALC are primarily associated with the autosomal recessive Krabbe's disease. This disease is characterized by developmental delay caused by apoptosis of myelin-forming cells. GALC is responsible for hydrolyzing galactosylceramide, a cerebroside that is an important component of myelin. A deficiency in GALC causes loss of myelin to nerve cells, resulting in delayed nerve transmissions. Krabbe's disease has varying degrees of severity due to a large number of different genetic mutations in the gene. The GALC antibody can be used to detect the deletions in the GALC gene and functions of the enzyme (PMID:20886637). Normal GALC mRNA encodes the 80 kDa precursor, which is processed into 50 and 30 kDa subunits (PMID: 26865610).