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GTSE1 Monoclonal antibody

GTSE1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3B4A12

Cat no : 68313-1-Ig

Synonyms

B99, G 2 and S phase expressed 1, GTSE 1, GTSE1, Protein B99 homolog

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using GTSE1 Monoclonal antibody (68313-1-Ig)

    WB analysis using 68313-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68313-1-Ig (GTSE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inU2OS cells, Saos-2 cells, HeLa cells, HEK-293 cells, MDA-MB-231 cells, Jurkat cells, K-562 cells, HSC-T6 cells, 4T1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68313-1-Ig targets GTSE1 in WB, ELISA applications and shows reactivity with Human, Rat, mouse samples.

Tested Reactivity Human, Rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GTSE1 fusion protein Ag32817
Full Name G-2 and S-phase expressed 1
Calculated Molecular Weight 77 kDa
Observed Molecular Weight67 kDa
GenBank Accession NumberBC006325
Gene Symbol GTSE1
Gene ID (NCBI) 51512
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GTSE1 is only expressed in the S and G2 phases of the cell cycle, where it colocalizes with cytoplasmic tubulin and microtubules. In response to DNA damage, the encoded protein accumulates in the nucleus and binds the tumor suppressor protein p53, shutting it out of the nucleus and repressing its ability to induce apoptosis.

Protocols

Product Specific Protocols
WB protocol for GTSE1 antibody 68313-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols