GTF2F2 Polyclonal antibody, PBS Only

GTF2F2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15139-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of K-562 using 15139-1-AP (same clone as 15139-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 15139-1-AP (same clone as 15139-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat spleen using 15139-1-AP (same clone as 15139-1-PBS)

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15139-1-AP (same clone as 15139-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 15139-1-AP (same clone as 15139-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15139-1-AP (same clone as 15139-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15139-1-PBS in a different storage buffer formulation.

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Product Information

15139-1-PBS targets GTF2F2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTF2F2 fusion protein Ag7313
• Full Name: general transcription factor IIF, polypeptide 2, 30kDa
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001771
• Gene Symbol: GTF2F2
• Gene ID (NCBI): 2963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.