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GTF2F2 Polyclonal antibody

GTF2F2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15139-1-AP

Synonyms

ATP dependent helicase GTF2F2, BTF4, GTF2F2, RAP30, TF2F2, TFIIF, TFIIF beta

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using GTF2F2 Polyclonal antibody (15139-1-AP)

    WB analysis of K-562 using 15139-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using GTF2F2 Polyclonal antibody (15139-1-AP)

    WB analysis of mouse spleen using 15139-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat spleen tissue using GTF2F2 Polyclonal antibody (15139-1-AP)

    WB analysis of rat spleen using 15139-1-AP

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GTF2F2 Polyclonal antibody (15139-1-AP)

    WB analysis of HeLa using 15139-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using GTF2F2 Polyclonal antibody (15139-1-AP)

    WB analysis of PC-3 using 15139-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15139-1-AP (GTF2F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GTF2F2 Polyclonal antibody (15139-1-AP)

    IF Staining of HeLa using 15139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15139-1-AP (GTF2F2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, mouse spleen tissue, PC-3 cells, rat spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15139-1-AP targets GTF2F2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2F2 fusion protein Ag7313
Full Name general transcription factor IIF, polypeptide 2, 30kDa
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC001771
Gene Symbol GTF2F2
Gene ID (NCBI) 2963
RRIDAB_2114554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GTF2F2 antibody 15139-1-APDownload protocol
IF protocol for GTF2F2 antibody 15139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols