CoraLite® Plus 488-conjugated GSTT1 Monoclonal antibody

GSTT1 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4C11

Cat no : CL488-67645

Synonyms

Glutathione transferase T1 1, GST class theta 1, GSTT1

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Validation Data Gallery

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  IF Staining of mouse liver using CL488-67645

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using CoraLite® Plus 488 GSTT1 antibody (CL488-67645, Clone: 1A4C11 ) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse liver tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67645 targets GSTT1 in IF-P applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSTT1 fusion protein Ag8573
• Full Name: glutathione S-transferase theta 1
• Calculated Molecular Weight: 240 aa, 27 kDa
• Observed Molecular Weight: 28 kDa, 30 kDa
• GenBank Accession Number: BC007065
• Gene Symbol: GSTT1
• Gene ID (NCBI): 2952
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GSTT1 belongs to the GST superfamily and Theta family. It is a conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. GSTT1 acts on 1,2-epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4-nitrobenzyl chloride and 4-nitrophenethyl bromide. It displays glutathione peroxidase activity with cumene hydroperoxide. GSTM1 and GSTT1 has been suggested as a risk factor in various cancers. GSTT1 is important in the detoxification of unidentified xenobiotics in the large intestine.