GSTM3 Monoclonal antibody, PBS Only

GSTM3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E2B8

Cat no : 67634-1-PBS

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

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Validation Data Gallery

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  WB analysis of HepG2 using 67634-1-Ig (same clone as 67634-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 67634-1-Ig (same clone as 67634-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67634-1-Ig (same clone as 67634-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67634-1-Ig (same clone as 67634-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GSTM3 antibody (67634-1-Ig, Clone: 1E2B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67634-1-PBS in a different storage buffer formulation.

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Product Information

67634-1-PBS targets GSTM3 in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig samples.

• Tested Reactivity: Human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSTM3 fusion protein Ag7494
• Full Name: glutathione S-transferase mu 3 (brain)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC000088
• Gene Symbol: GSTM3
• Gene ID (NCBI): 2947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.