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GSTM3 Monoclonal antibody

GSTM3 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig and More (1)

Applications

WB, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1E2B8

Cat no : 67634-1-Ig

Synonyms

Glutathione S transferase Mu 3, GST class mu 3, GST5, GSTB, GSTM3, GSTM3 3, GTM3, hGSTM3 3

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of HepG2 using 67634-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of pig brain using 67634-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    WB analysis of HeLa using 67634-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67634-1-Ig (GSTM3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using GSTM3 Monoclonal antibody (67634-1-Ig)

    IF Staining of A431 using 67634-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using GSTM3 antibody (67634-1-Ig, Clone: 1E2B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, pig brain tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67634-1-Ig targets GSTM3 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, pig samples.

Tested Reactivity Human, pig
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTM3 fusion protein Ag7494
Full Name glutathione S-transferase mu 3 (brain)
Calculated Molecular Weight 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC000088
Gene Symbol GSTM3
Gene ID (NCBI) 2947
RRIDAB_2882835
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GSTM3 is a cytosolic enzyme involved in prostaglandin and leukotriene synthesis and in the metabolization of toxic compounds, such as chemotherapeutic drugs, insecticides, herbicides, carcinogens, and by-products of oxidative stress. GSTM3 is expressed in testis, brain, lung, and lymphocytes. GSTM3 have been reported to be dysregulated in cancers like prostate cancer and colon cancer.

Protocols

Product Specific Protocols
WB protocol for GSTM3 antibody 67634-1-IgDownload protocol
IF protocol for GSTM3 antibody 67634-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF,CoIP

Reprod Biol Endocrinol

A loss-of-function variant in SSFA2 causes male infertility with globozoospermia and failed oocyte activation.

Authors - Gelin Huang
View Article