GSTM1 Polyclonal antibody, PBS Only

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  WB analysis using 12412-1-AP (same clone as 12412-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse kidney using 12412-1-AP (same clone as 12412-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 12412-1-AP (same clone as 12412-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human gliomas using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 12412-1-AP (same clone as 12412-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12412-1-AP (same clone as 12412-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12412-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12412-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.5.1.18, glutathione S transferase mu 1, Glutathione S-transferase Mu 1, GST class mu 1, GST class-mu 1

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Product Information

12412-1-PBS targets GSTM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM1 fusion protein Ag3113
• Full Name: glutathione S-transferase mu 1
• Calculated Molecular Weight: 181 aa, 21 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC024005
• Gene Symbol: GSTM1
• Gene ID (NCBI): 2944
• RRID: AB_2115925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P09488
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

GSTM1 belongs to the superfamily of glutathione-Stransferases (GSTs) of phase 2 enzymes that catalyze a number of distinct glutathione-dependent reactions. GSTM1 gene has been a natural candidate in studies of susceptibility to inflammatory airway diseases with environmental components because of the high prevalence of the null polymorphism and its role in detoxification (PMID: 22683820).