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  • KD/KO Validated

GSTM1 Polyclonal antibody

GSTM1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12412-1-AP

Synonyms

glutathione S transferase mu 1, GST class mu 1, GST HB subunit 4, GST1, GSTM1, GSTM1 1, GSTM1a 1a, GSTM1b 1b, GTH4, GTM1, H B, MU, MU 1



Tested Applications

Positive WB detected inmouse liver tissue, HeLa cells, mouse ovary tissue, mouse kidney tissue, rat liver
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12412-1-AP targets GSTM1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GSTM1 fusion protein Ag3113
Full Name glutathione S-transferase mu 1
Calculated molecular weight 181 aa, 21 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC024005
Gene symbol GSTM1
Gene ID (NCBI) 2944
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTM1 antibody 12412-1-APDownload protocol
IHC protocol for GSTM1 antibody 12412-1-APDownload protocol
IF protocol for GSTM1 antibody 12412-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Immunity

Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.

Authors - Yujia Wang
  • KO Validated
humanIHC

Acta Neuropathol

Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy.

Authors - Woutje M Berdowski
mouseWB

Sci Adv

lncRNA-GM targets Foxo1 to promote T cell-mediated autoimmunity

Authors - Yali Chen
humanWB

Autophagy

ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.

Authors - Zhiqiang Deng
humanWB

Virol Sin

Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.

Authors - Yingying Song
mouseWB

Free Radic Biol Med

Activation of PPARα-catalase pathway reverses alcoholic liver injury via upregulating NAD synthesis and accelerating alcohol clearance.

Authors - Ruichao Yue