GSTM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSTM1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

22

Reviews (1)

Cat no : 12412-1-AP

Synonyms

GST HB subunit 4, GST class-mu 1, GST class mu 1, Glutathione S-transferase Mu 1, glutathione S transferase mu 1

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Validation Data Gallery

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  WB analysis using 12412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12412-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12412-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12412-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12412-1-AP (GSTM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 12412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 12412-1-AP (GSTM1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12412-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GSTM1 antibody (12412-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse ovary tissue, HeLa cells, mouse kidney tissue, rat liver
• Positive IHC detected in: mouse liver tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

12412-1-AP targets GSTM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSTM1 fusion protein Ag3113
• Full Name: glutathione S-transferase mu 1
• Calculated Molecular Weight: 181 aa, 21 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC024005
• Gene Symbol: GSTM1
• Gene ID (NCBI): 2944
• RRID: AB_2115925
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang ; KO Validated
human	WB	Mol Cell; SMURF2 predisposes cancer cell toward ferroptosis in GPX4-independent manners by promoting GSTP1 degradation; Authors - Wenxin Zhang
human	IHC	Acta Neuropathol; Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy.; Authors - Woutje M Berdowski
mouse	WB	Sci Adv; lncRNA-GM targets Foxo1 to promote T cell-mediated autoimmunity; Authors - Yali Chen
human	WB	Autophagy; ALS-FTLD-linked mutations of SQSTM1/p62 disrupt selective autophagy and NFE2L2/NRF2 anti-oxidative stress pathway.; Authors - Zhiqiang Deng
human	WB	Virol Sin; Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection.; Authors - Yingying Song

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (01-26-2024)

Antibody works well at provided dilution in 1X TBST. Bands showed up around 25 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse liver tissue