GSTA1 Monoclonal antibody, PBS Only

GSTA1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1F4

Cat no : 66624-1-PBS

Synonyms

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

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Validation Data Gallery

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  WB analysis of pig liver using 66624-1-Ig (same clone as 66624-1-PBS)

  pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 66624-1-Ig (same clone as 66624-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66624-1-Ig (same clone as 66624-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 66624-1-Ig (same clone as 66624-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human liver using 66624-1-Ig (same clone as 66624-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat kidney using 66624-1-Ig (same clone as 66624-1-PBS)

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse hepatocytes using 66624-1-Ig (same clone as 66624-1-PBS)

  mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66624-1-Ig (same clone as 66624-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66624-1-Ig (same clone as 66624-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66624-1-Ig (same clone as 66624-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66624-1-Ig (same clone as 66624-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66624-1-Ig (same clone as 66624-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66624-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66624-1-PBS targets GSTA1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSTA1 fusion protein Ag6084
• Full Name: glutathione S-transferase alpha 1
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC053578
• Gene Symbol: GSTA1
• Gene ID (NCBI): 2938
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.