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GSTA1 Monoclonal antibody

GSTA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1F4

Cat no : 66624-1-Ig

Synonyms

Glutathione S transferase A1, GST class alpha member 1, GST epsilon, GST HA subunit 1, GST2, GSTA1, GSTA1 1, GTH1

Validation Data Gallery

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  • Western Blot (WB) analysis of pig liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of pig liver using 66624-1-Ig

    pig liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of mouse liver using 66624-1-Ig

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of rat liver using 66624-1-Ig

    rat liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of human testis using 66624-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of human liver using 66624-1-Ig

    human liver tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of rat kidney using 66624-1-Ig

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse hepatocytes using GSTA1 Monoclonal antibody (66624-1-Ig)

    WB analysis of mouse hepatocytes using 66624-1-Ig

    mouse hepatocytes were subjected to SDS PAGE followed by western blot with 66624-1-Ig (GSTA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human kidney using 66624-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human kidney using 66624-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human liver cancer using 66624-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GSTA1 Monoclonal antibody (66624-1-Ig)

    IHC staining of human liver cancer using 66624-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66624-1-Ig (GSTA1 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GSTA1 Monoclonal antibody (66624-1-Ig)

    IF Staining of HepG2 using 66624-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66624-1-Ig (GSTA1 antibody) at dilution of 1:200 and Alexa Fluor 488-conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inpig liver tissue, human liver tissue, rat kidney tissue, mouse hepatocytes, human testis tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman kidney tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

66624-1-Ig targets GSTA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Cited Reactivityhuman, mouse, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen GSTA1 fusion protein Ag6084
Full Name glutathione S-transferase alpha 1
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC053578
Gene Symbol GSTA1
Gene ID (NCBI) 2938
RRIDAB_2881984
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GSTA1 antibody 66624-1-IgDownload protocol
IHC protocol for GSTA1 antibody 66624-1-IgDownload protocol
IF protocol for GSTA1 antibody 66624-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Anim Physiol Anim Nutr (Berl)

Tolerable upper intake level of iron damages the liver of weaned piglets.

Authors - Haoxuan Ding
View Article
human,mouseWB

Int J Mol Sci

Upregulation of Hepatic Glutathione S-Transferase Alpha 1 Ameliorates Metabolic Dysfunction-Associated Steatosis by Degrading Fatty Acid Binding Protein 1

Authors - Jing Jiang
View Article