ChromoTek GST-Trap Agarose, kit

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

Specificity

GST

Applications

IP, CoIP, ChIP, RIP

Conjugate

Agarose

Type

Nanobody

Cat no : stak

Synonyms

GST-Trap, GST, GST tag, Glutathione S transferase

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Validation Data Gallery

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  Pull-down of GST with GST-Trap Agarose: Coomassie and Western blot; Immunoprecipitation of GST with GST-Trap. Note effective pull-down of GST-fusion proteins: No GST-fusions left in non-bound (FT) lane in Western Blot. I: Input, FT: Flow-Through, B: Bound.

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Product Information

GST-Trap is an affinity resin for IP of GST fusion proteins. It consists of a GST Nanobody/ VHH coupled to agarose beads.

• Description: The GST-Trap Agarose Kit contains GST-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of GST-fusion proteins and their interacting factors.

  • Effectively immunoprecipitates low expressed GST-fusion proteins

  • Considerably higher affinity than glutathione cellulose and other affinity resins

  • Strong binding even under harsh washing conditions

  • No heavy & light chains in your downstream application

• Applications: IP, CoIP, ChIP, RIP
• Specificity/Target: Glutathione S-Transferase (GST) from Schistosoma japonicum
• Binding capacity: 7.5-10 μg of recombinant GST per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.2 M glycine pH 2.5
• Wash buffer compatibility: 4 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 1 nM
• Compatibility with mass spectrometry: The GST-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2631414
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

Component	Description
GST-Trap Agarose	20 reactions (500 µL)
Lysis buffer	Optimized for cytoplasmatic proteins and mammalian cell lysis
RIPA buffer	Optimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer	Removal of unwanted proteins, peptides, etc.
Dilution buffer	Dilution of cell lysate
Elution buffer	For acidic elution

Other Formats

GST-Trap Agarose