CoraLite® Plus 488-conjugated GSNOR,ADH5 Polyclonal antibody

• Featured Product
• KD/KO Validated

GSNOR,ADH5 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16379

Synonyms

ADH5, Alcohol dehydrogenase class-3, ADHX, ADH3, ADH 5

---

Validation Data Gallery

• 

  FC experiment of HepG2 using CL488-16379

  1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated GSNOR,ADH5 Polyclonal antibody (CL488-16379)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-16379 targets GSNOR,ADH5 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GSNOR,ADH5 fusion protein Ag9360
• Full Name: alcohol dehydrogenase 5 (class III), chi polypeptide
• Calculated Molecular Weight: 374 aa, 40 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC014665
• Gene Symbol: ADH5
• Gene ID (NCBI): 128
• RRID: AB_2919133
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ADH5, also named as ADHX, FDH FALDH GSH-FDH ADH3 and GSNOR, belongs to the zinc-containing alcohol dehydrogenase family and Class-III subfamily. It is remarkably ineffective in oxidizing ethanol, but it readily catalyzes the oxidation of long-chain primary alcohols and the oxidation of S-(hydroxymethyl) glutathione. ADH5 mediates multiple cardiovascular functions. It plays in regulating heterocellular communication in the artery wall. (PMID:21071693). ADH5 immunostaining is distributed in both the nucleus and cytoplasm of the retinal ganglion cells(PMID:22117533).

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C