GSK3B Recombinant antibody

GSK3B Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

4N21

Cat no : 82061-1-RR

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 82061-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 82061-1-RR

  IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug.

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  IHC staining of mouse skin using 82061-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 82061-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82061-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82061-1-RR against Human GSK3B were performed. The affinity constant is 7.36 pM.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, A549 cells, Jurkat cells, PC-12 cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:225-1:900

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82061-1-RR targets GSK3B in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSK3B fusion protein Ag17320
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: ~45 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.