GSK3B Recombinant antibody, PBS Only

GSK3B Uni-rAb^TM Recombinant Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4N21

Cat no : 82061-1-PBS

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 82061-1-RR (same clone as 82061-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82061-1-RR (GSK3B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82061-1-RR (same clone as 82061-1-PBS)

  IP result of anti-GSK3B (IP:82061-1-RR, 4ug; Detection:82061-1-RR 1:5000) with HeLa cells lysate 1720 ug. This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skin using 82061-1-RR (same clone as 82061-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 82061-1-RR (GSK3B antibody) at dilution of 1:450 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82061-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82061-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82061-1-RR against Human GSK3B were performed. The affinity constant is 7.36 pM.

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Product Information

82061-1-PBS targets GSK3B in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSK3B fusion protein Ag17320
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: ~45 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase.GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. In skeletal muscle, it contributes to INS regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis.