GSK3B Monoclonal antibody

GSK3B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (2)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

2E9E4

Cat no : 67329-1-Ig

Synonyms

GSK3 beta, GSK-3 beta, GSK 3 beta, EC:2.7.11.26, EC:2.7.11.1

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Validation Data Gallery

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  WB analysis using 67329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67329-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 67329-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67329-1-Ig (GSK3B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67329-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67329-1-Ig (GSK3B antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 67329-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GSK3B antibody (67329-1-Ig, Clone: 2E9E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, pig brain tissue, HEK-293 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, MOLT-4
• Positive IHC detected in: human breast cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 10 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

67329-1-Ig targets GSK3B in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GSK3B fusion protein Ag17320
• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 46-48 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• RRID: AB_2882588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase .GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation.In skeletal muscle, it contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Researches showed that the crystal structure of human GSK3B, expressed in insect cells, at 2.8-angstrom resolution .

Publications

Species	Application	Title
mouse	WB	Mol Nutr Food Res; Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice; Authors - Songfeng Yu
human	WB,IF	J Inflamm Res; Exogenous Plant gma-miR-159a, Identified by miRNA Library Functional Screening, Ameliorated Hepatic Stellate Cell Activation and Inflammation via Inhibiting GSK-3β-Mediated Pathways.; Authors - Wen-Ying Yu
mouse	IF	Neurochem Res; Aerobic Exercise Ameliorates Cognitive Disorder and Declined Oxidative Stress via Modulating the Nrf2 Signaling Pathway in D-galactose Induced Aging Mouse Model; Authors - Guangjing Xie
human	WB	Environ Toxicol; Inhibition of FAM83D displays antitumor effects in glioblastoma via down-regulation of the AKT/Wnt/β-catenin pathway.; Authors - Jubo Wang
human	WB,CoIP	J Clin Lab Anal; Circular RNA circβ-catenin aggravates the malignant phenotype of non-small-cell lung cancer via encoding a peptide.; Authors - Weijun Zhao
human	WB	Biomed Res Int; Emodin Alleviates Hydrogen Peroxide-Induced Inflammation and Oxidative Stress via Mitochondrial Dysfunction by Inhibiting the PI3K/mTOR/GSK3β Pathway in Neuroblastoma SH-SY5Y Cells.; Authors - Rui Li