GSDMA Recombinant antibody

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  WB analysis using 82920-2-RR

  Daudi cells were subjected to SDS PAGE followed by western blot with 82920-2-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 82920-2-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMA antibody (82920-2-RR, Clone: 230161C5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  Affinity and Kinetic Characterization of 82920-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82920-2-RR against Human GSDMA were performed. The affinity constant is below 1 pM.

GSDMA {ptg:Brand} Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82920-2-RR

Clone No.230161C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9

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Tested Applications

• Positive WB detected in: Daudi cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82920-2-RR targets GSDMA in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSDMA fusion protein Ag24407
• Full Name: gasdermin A
• Calculated Molecular Weight: 445 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC109197
• Gene Symbol: GSDMA
• Gene ID (NCBI): 284110
• RRID: AB_3670656
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96QA5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.