GSDMA Recombinant antibody, PBS Only

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  WB analysis using 82920-2-RR (same clone as 82920-2-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 82920-2-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82920-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82920-2-RR (same clone as 82920-2-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMA antibody (82920-2-RR, Clone: 230161C5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82920-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82920-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82920-2-RR against Human GSDMA were performed. The affinity constant is below 1 pM.

GSDMA {ptg:Brand} Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82920-2-PBS

Clone No.230161C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9

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Product Information

82920-2-PBS targets GSDMA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSDMA fusion protein Ag24407
• Full Name: gasdermin A
• Calculated Molecular Weight: 445 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC109197
• Gene Symbol: GSDMA
• Gene ID (NCBI): 284110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q96QA5
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.