GSDMA Recombinant antibody

GSDMA Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230161D4

Cat no : 82920-1-RR

Synonyms

Gasdermin-A, Gasdermin-1, gasdermin A, Gasdermin 1, FKSG9

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Validation Data Gallery

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  WB analysis of Daudi using 82920-1-RR

  Daudi cells were subjected to SDS PAGE followed by western blot with 82920-1-RR (GSDMA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 82920-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82920-1-RR against Human GSDMA were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82920-1-RR targets GSDMA in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GSDMA fusion protein Ag24407
• Full Name: gasdermin A
• Calculated Molecular Weight: 445 aa, 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC109197
• Gene Symbol: GSDMA
• Gene ID (NCBI): 284110
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gasdermins, a family of five pore-forming proteins (GSDMA-GSDME) in humans expressed predominantly in the skin, mucosa and immune sentinel cells, are key executioners of inflammatory cell death (pyroptosis), which recruits immune cells to infection sites and promotes protective immunity. GSDMA was predicted to enable phosphatidylinositol-4,5-bisphosphate binding activity, phosphatidylinositol-4-phosphate binding activity, and phosphatidylserine binding activity. Involved in apoptotic process.