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CoraLite®555-conjugated GRP94 Polyclonal antibody

GRP94 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-14700

Synonyms

HSP90B1, Heat shock protein family C member 4, GP96, Endoplasmin, ECGP

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite®555-conjugated GRP94 Polyclonal antibody (CL555-14700)

    IF Staining of HeLa using CL555-14700

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 GRP94 antibody (CL555-14700) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite®555-conjugated GRP94 Polyclonal antibody (CL555-14700)

    FC experiment of HeLa using CL555-14700

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human GRP94 (CL555-14700) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-14700 targets GRP94 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GRP94 fusion protein Ag6344
Full Name heat shock protein 90kDa beta (Grp94), member 1
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC066656
Gene Symbol GRP94
Gene ID (NCBI) 7184
RRIDAB_2919619
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Glucose-regulated protein 94 (GRP94), also called HSP90B1 and GP96, is an endoplasmic reticulum (ER)-resident member of the heat shock protein 90 (HSP90) family (PMID: 33802964; 24658275). Under ER stress conditions, GRP94 accelerates its function as a molecular chaperone. Client proteins of GRP94 include toll-like receptors (TLRs), glycoprotein (GP) IX subunit, insulin-like growth factors (IGFs), proinsulin, and integrins (PMID: 7913987; 32781621; 22079671). As well as being an ER chaperone, GRP94 can also participate in Calcium regulation and is also a regulator of innate and adaptive immunity (PMID: 33802964; 11584270).

Protocols

Product Specific Protocols
IF protocol for CL555 GRP94 antibody CL555-14700Download protocol
FC protocol for CL555 GRP94 antibody CL555-14700Download protocol
Standard Protocols
Click here to view our Standard Protocols