NMDAR1/GRIN1 Monoclonal antibody, PBS Only

NMDAR1/GRIN1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1H2C2

Cat no : 67717-1-PBS

Synonyms

GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1

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Validation Data Gallery

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  WB analysis using 67717-1-Ig (same clone as 67717-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67717-1-Ig (same clone as 67717-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 67717-1-Ig (same clone as 67717-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (67717-1-Ig, Clone: 1H2C2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67717-1-PBS in a different storage buffer formulation.

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Product Information

67717-1-PBS targets NMDAR1/GRIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26364
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105-120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: NMDAR1
• Gene ID (NCBI): 2902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.