NMDAR1/GRIN1 Monoclonal antibody

NMDAR1/GRIN1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H2C2

Cat no : 67717-1-Ig

Synonyms

GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1



Tested Applications

Positive WB detected inpig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

67717-1-Ig targets NMDAR1/GRIN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

Tested Reactivity human, mouse, rat, rabbit, chicken
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen NMDAR1/GRIN1 fusion protein Ag26364
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight105120 kDa
GenBank Accession NumberNM_000832
Gene Symbol NMDAR1
Gene ID (NCBI) 2902
RRIDAB_2882906
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for NMDAR1/GRIN1 antibody 67717-1-IgDownload protocol
IHC protocol for NMDAR1/GRIN1 antibody 67717-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Brain Res

The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults

Authors - Bei Guo
mouse

Heliyon

A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice

Authors - Hanyu Luo
ratWB

Biomedicines

Impaired Response to Mismatch Novelty in the Li2+-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs

Authors - Carlos Nascimento