NMDAR1/GRIN1 Monoclonal antibody

NMDAR1/GRIN1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, chicken

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

3

CloneNo.

1H2C2

Cat no : 67717-1-Ig

Synonyms

GRIN1, NMDAR1, NMDAR 1, Glutamate receptor ionotropic, NMDA 1, GluN1

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Validation Data Gallery

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  WB analysis using 67717-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit cerebellum using 67717-1-Ig

  rabbit cerebellum tissue were subjected to SDS PAGE followed by western blot with 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse cerebellum using 67717-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 67717-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67717-1-Ig (NMDAR1/GRIN1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 67717-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using NMDAR1/GRIN1 antibody (67717-1-Ig, Clone: 1H2C2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: pig brain tissue, rabbit cerebellum tissue, rat brain tissue, mouse brain tissue, chicken brain tissue
• Positive IHC detected in: mouse cerebellum tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

67717-1-Ig targets NMDAR1/GRIN1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, rabbit, chicken
• Cited Reactivity: mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: NMDAR1/GRIN1 fusion protein Ag26364
• Full Name: glutamate receptor, ionotropic, N-methyl D-aspartate 1
• Calculated Molecular Weight: 105 kDa
• Observed Molecular Weight: 105-120 kDa
• GenBank Accession Number: NM_000832
• Gene Symbol: NMDAR1
• Gene ID (NCBI): 2902
• RRID: AB_2882906
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Exp Brain Res; The NR2B-targeted intervention alleviates the neuronal injuries at the sub-acute stage of cerebral ischemia: an exploration of stage-dependent strategy against ischemic insults; Authors - Bei Guo
mouse		Heliyon; A peptide from the Japanese encephalitis virus failed to induce the production of anti-N-methyl-d-aspartate receptor antibodies via molecular mimicry in mice; Authors - Hanyu Luo
rat	WB	Biomedicines; Impaired Response to Mismatch Novelty in the Li2+-Pilocarpine Rat Model of TLE: Correlation with Hippocampal Monoaminergic Inputs; Authors - Carlos Nascimento