GRASP65,GORASP1 Monoclonal antibody, PBS Only

GRASP65,GORASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2C10

Cat no : 66651-1-PBS

Synonyms

GORASP1, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1, Golgi phosphoprotein 5

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Validation Data Gallery

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  WB analysis using 66651-1-Ig (same clone as 66651-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66651-1-Ig (same clone as 66651-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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  IF Staining of SK-BR-3 using 66651-1-Ig (same clone as 66651-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

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Product Information

66651-1-PBS targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRASP65,GORASP1 fusion protein Ag1054
• Full Name: golgi reassembly stacking protein 1, 65kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC008928
• Gene Symbol: GRASP65
• Gene ID (NCBI): 64689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.