Filter: AllWBIHCIF/ICC

WB analysis using 66651-1-Ig (same clone as 66651-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
WB analysis of HSC-T6 using 66651-1-Ig (same clone as 66651-1-PBS)
HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 66651-1-Ig (same clone as 66651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
IHC staining of human stomach cancer using 66651-1-Ig (same clone as 66651-1-PBS)
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.
IF Staining of SK-BR-3 using 66651-1-Ig (same clone as 66651-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66651-1-PBS in a different storage buffer formulation.

GRASP65,GORASP1 Monoclonal antibody, PBS Only

GRASP65,GORASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 66651-1-PBS

Clone No.1B2C10

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GORASP1, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1, Golgi phosphoprotein 5

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66651-1-PBS targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	GRASP65,GORASP1 fusion protein Ag1054 Predict reactive species
Full Name	golgi reassembly stacking protein 1, 65kDa
Calculated Molecular Weight	46 kDa
Observed Molecular Weight	65-70 kDa
GenBank Accession Number	BC008928
Gene Symbol	GRASP65
Gene ID (NCBI)	64689
RRID	AB_2882008
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BQQ3
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.