GRASP65,GORASP1 Monoclonal antibody

GRASP65,GORASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

1B2C10

Cat no : 66651-1-Ig

Synonyms

GORASP1, GOLPH5, Golgi reassembly-stacking protein of 65 kDa, Golgi reassembly-stacking protein 1, Golgi phosphoprotein 5

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Validation Data Gallery

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  WB analysis using 66651-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 66651-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 66651-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66651-1-Ig (GRASP65,GORASP1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SK-BR-3 using 66651-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed SK-BR-3 cells using GRASP65,GORASP1 antibody (66651-1-Ig, Clone: 1B2C10 ) at dilution of 1:3876 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Tested Applications

• Positive WB detected in: HeLa cells, HSC-T6 cells, HEK-293 cells, MCF-7 cells, Jurkat cells
• Positive IHC detected in: human prostate cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SK-BR-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1938-1:7752

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

66651-1-Ig targets GRASP65,GORASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GRASP65,GORASP1 fusion protein Ag1054
• Full Name: golgi reassembly stacking protein 1, 65kDa
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC008928
• Gene Symbol: GRASP65
• Gene ID (NCBI): 64689
• RRID: AB_2882008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Golgi reassembly-stacking protein 1 is a protein that in humans is encoded by the GORASP1 gene. The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.; Authors - Shuai Li
human	WB	Mol Biol Cell; Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking.; Authors - Stephen C Ireland
human	WB	J Cell Biochem; Golgi apparatus fragmentation participates in oxidized low-density lipoprotein-induced endothelial cell injury.; Authors - Guang Ji
human	WB	Cells; Discovery of Cell Number-Interstitial Fluid Volume (CIF) Ratio Reveals Secretory Autophagy Pathway to Supply eHsp90α for Wound Healing; Authors - Cheng Chang ; KO Validated