GPX4 (Human Specific) Recombinant antibody

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  WB analysis using 82822-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82822-2-RR

  WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells.

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  Affinity and Kinetic Characterization of 82822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

GPX4 (Human Specific) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 82822-2-RR

Clone No.6E9

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:15000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82822-2-RR targets GPX4 (Human Specific) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPX4 (Human Specific) fusion protein Ag30650
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC021567
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• RRID: AB_3086547
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36969
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.

Publications

Species	Application	Title
human	WB	Biochem Pharmacol; HDAC inhibitors activate lipid peroxidation and ferroptosis in gastric cancer; Authors - Robert Jenke