GPX4 (Human Specific) Recombinant antibody, PBS Only

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  WB analysis using 82822-2-RR (same clone as 82822-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82822-2-RR (GPX4 antibody) at dilution of 1:7500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 82822-2-RR (same clone as 82822-2-PBS)

  WB result of GPX4 (Human Specific) antibody (82822-2-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GPX4 (Human Specific) transfected HEK-293 cells. This data was developed using the same antibody clone with 82822-2-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82822-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82822-2-RR against Human GPX4 (Human Specific) were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

GPX4 (Human Specific) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 82822-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

GPX4, GPx-4, GPx 4, EC:1.11.1.9, EC:1.11.1.12

Formulation:  PBS Only

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Size/Concentration: 

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Freight/Packing: -

Quantity

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Product Information

82822-2-PBS targets GPX4 (Human Specific) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GPX4 (Human Specific) fusion protein Ag30650
• Full Name: glutathione peroxidase 4 (phospholipid hydroperoxidase)
• Observed Molecular Weight: 20-23 kDa
• GenBank Accession Number: BC021567
• Gene Symbol: GPX4
• Gene ID (NCBI): 2879
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P36969
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

GPX4 (Phospholipid hydroperoxide glutathione peroxidase, mitochondrial) protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility.It has two isforms about 20KDa and 22KDa, respectively. GPX4 is a monomer,but it has a tendency to form higher mass oligomers (PMID:17630701).82822-2-RR is human specific.