CoraLite® Plus 488-conjugated GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F6B5

Cat no : CL488-67531

Synonyms

AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1

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Validation Data Gallery

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  IF Staining of HepG2 using CL488-67531

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 GPT/ALT1 antibody (CL488-67531, Clone: 5F6B5 ) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67531 targets GPT/ALT1 in IF/ICC applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: GPT/ALT1 fusion protein Ag10453
• Full Name: glutamic-pyruvate transaminase (alanine aminotransferase)
• Calculated Molecular Weight: 496 aa, 55 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC018207
• Gene Symbol: ALT1
• Gene ID (NCBI): 2875
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.