CoraLite®594-conjugated GPR37/Pael-R Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR37/Pael-R Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-14820

Synonyms

EDNRBL, ETBR LP 1, GPR37, hET(B)R LP, PAELR

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Validation Data Gallery

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  IF Staining of mouse brain using CL594-14820

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GPR37/Pael-R antibody (CL594-14820) at dilution of 1:200.

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  IF Staining of mouse brain using CL594-14820

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite®594 GPR37/Pael-R antibody (CL594-14820) at dilution of 1:200.

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Tested Applications

• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-14820 targets GPR37/Pael-R in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR37/Pael-R fusion protein Ag6591
• Full Name: G protein-coupled receptor 37 (endothelin receptor type B-like)
• Calculated Molecular Weight: 67 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC040007
• Gene Symbol: GPR37
• Gene ID (NCBI): 2861
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The orphan G protein-coupled receptor GPR37, also known as PAELR (parkin-associated endothelin receptor-like receptor) or ETBR-LP-1 (endothelin B receptor-like protein 1), is a 613 aa, multi-pass membrane protein predominantly expressed in the brain. It is a substrate of parkin (PARK2). When overexpressed in cells, GPR37 tends to become insoluble, unfolded, and ubiquitinated in vivo. Accumulation of the unfolded protein may lead to dopaminergic neuronal death in juvenile Parkinson disease (JPD). This antibody recognizes endogenous GPR37, which migrates as an approximately 50-55 kDa band in SDS-PAGE (PMID:17519329).