GPR161 Polyclonal antibody

• Featured Product
• KD/KO Validated

GPR161 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (4)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

39

Reviews (2)

Cat no : 13398-1-AP

Synonyms

G-protein coupled receptor RE2, G-protein coupled receptor 161

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Validation Data Gallery

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  WB analysis using 13398-1-AP

  mouse brain tissue was subjected to SDS PAGE followed by western blot with 13398-1-AP (GPR161 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of MDCK using 13398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using GPR161 antibody (13398-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

• 

  IF Staining of ARPE-19 using 13398-1-AP

  Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using GPR161 antibody (13398-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

• 

  IF Staining of C2C12 using 13398-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using GPR161 antibody (13398-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IF/ICC detected in: MDCK cells, ARPE-19 cells, C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 34 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13398-1-AP targets GPR161 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, pig, chicken, zebrafish, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GPR161 fusion protein Ag4221
• Full Name: G protein-coupled receptor 161
• Calculated Molecular Weight: 529 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC028163
• Gene Symbol: GPR161
• Gene ID (NCBI): 23432
• RRID: AB_2113965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).

Publications

Species	Application	Title
zebrafish	IF	Cell; Vertebrate cells differentially interpret ciliary and extraciliary cAMP.; Authors - Melissa E Truong
chicken	IF	Sci Adv; Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.; Authors - Gabriela Toro-Tapia
human	IF	Sci Adv; Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.; Authors - Chuan Chen
mouse	IF	Nat Commun; Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.; Authors - Shi-Rong Hong
mouse	IF	Dev Cell; Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.; Authors - Francesc R Garcia-Gonzalo
mouse	WB,IF	EMBO J; Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.; Authors - Shichao Duan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (06-02-2023)

This protein is expressed within the primary cilia in starvation medium and exits it when stimulated with ShhN. I tested this antibody by IF on 3T3 but difficult to get some signal out. I switched to RPE1 cells that gave me nice signal if you permeabilise with triton and not Methanol. However, the signal is aspecific because there is no difference between - and + ShhN. I also tested at 6h and 24h post stimulation (even though it is reported to exit the primary cilia within 2h) with the same result : ciliary signal in both case.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50
• Cell Tissue Type: RPE1

GPR161 Antibody Immunofluorescence validation (1/50 dilution) in RPE1 (Cat no:13398-1-AP)

FH

daniel (Verified Customer) (09-15-2022)

very efficient

• Applications: Western Blot

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