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GPR161 Polyclonal antibody

GPR161 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (4)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13398-1-AP

Synonyms

G-protein coupled receptor RE2, G-protein coupled receptor 161



Tested Applications

Positive WB detected inmouse brain tissue
Positive IF/ICC detected inMDCK cells, ARPE-19 cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13398-1-AP targets GPR161 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse, pig, chicken, zebrafish, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPR161 fusion protein Ag4221
Full Name G protein-coupled receptor 161
Calculated Molecular Weight 529 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028163
Gene Symbol GPR161
Gene ID (NCBI) 23432
RRIDAB_2113965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).

Protocols

Product Specific Protocols
WB protocol for GPR161 antibody 13398-1-APDownload protocol
IF protocol for GPR161 antibody 13398-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
zebrafishIF

Cell

Vertebrate cells differentially interpret ciliary and extraciliary cAMP.

Authors - Melissa E Truong
chickenIF

Sci Adv

Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.

Authors - Gabriela Toro-Tapia
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
mouseIF

Nat Commun

Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.

Authors - Shi-Rong Hong
mouseIF

Dev Cell

Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.

Authors - Francesc R Garcia-Gonzalo
mouseWB,IF

EMBO J

Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.

Authors - Shichao Duan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Charlotte (Verified Customer) (06-02-2023)

This protein is expressed within the primary cilia in starvation medium and exits it when stimulated with ShhN. I tested this antibody by IF on 3T3 but difficult to get some signal out. I switched to RPE1 cells that gave me nice signal if you permeabilise with triton and not Methanol. However, the signal is aspecific because there is no difference between - and + ShhN. I also tested at 6h and 24h post stimulation (even though it is reported to exit the primary cilia within 2h) with the same result : ciliary signal in both case.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/50
  • Cell Tissue Type: RPE1
GPR161 Antibody Immunofluorescence validation (1/50 dilution) in RPE1 (Cat no:13398-1-AP)
FH

daniel (Verified Customer) (09-15-2022)

very efficient

  • Applications: Western Blot