|Positive WB detected in||mouse brain tissue|
|Positive IF detected in||C2C12 cells, MDCK cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
13398-1-AP targets GPR161 in WB, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.
|Tested Reactivity||human, mouse, Canine|
|Cited Reactivity||human, mouse, chicken, zebrafish, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GPR161 fusion protein Ag4221|
|Full Name||G protein-coupled receptor 161|
|Calculated molecular weight||529 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC028163|
|Gene ID (NCBI)||23432|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
GPR161 (also known as RE2) is an orphan G protein-coupled receptor and plays an important role in the Hh pathway. In the absence of Hh signals, GPR161 localizes to primary cilia and keeps the downstream GLI transcription factors in their repressor forms (PMID: 26305592). Ciliary localization of GPR161 requires TULP3 and the IFT-A complex. In the presence of SHH, GPR161 is removed from primary cilia and is internalized into recycling endosomes, preventing its activity and allowing activation of the Shh signaling. GPR161 has a calculated molecular mass of 59 kDa. The 70-kDa band probably represents a modified version of GPR161 (PMID: 18250320).
Vertebrate cells differentially interpret ciliary and extraciliary cAMP.
Primary cilium remodeling mediates a cell signaling switch in differentiating neurons.
Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.
Spatiotemporal manipulation of ciliary glutamylation reveals its roles in intraciliary trafficking and Hedgehog signaling.
Phosphoinositides Regulate Ciliary Protein Trafficking to Modulate Hedgehog Signaling.
Rabl2 GTP hydrolysis licenses BBSome-mediated export to fine-tune ciliary signaling.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (06-02-2023)
This protein is expressed within the primary cilia in starvation medium and exits it when stimulated with ShhN. I tested this antibody by IF on 3T3 but difficult to get some signal out. I switched to RPE1 cells that gave me nice signal if you permeabilise with triton and not Methanol. However, the signal is aspecific because there is no difference between - and + ShhN. I also tested at 6h and 24h post stimulation (even though it is reported to exit the primary cilia within 2h) with the same result : ciliary signal in both case.
daniel (Verified Customer) (09-15-2022)